The procedure offers a simple, accessible theoretical tool to identify cost-effective production strategies using bioreactors. IPTG and temperatures around 32 ☌ led to shorter cultures and lower PspA4Pro production costs. When the price of peptone was above the threshold value of US$ 30/kg, defined medium became the best choice. allel expression and purification of large numbers of proteins1-4, and these. Background Plant bioreactor offers an efficient and economical system for large-scale production of recombinant proteins. The core expenses were related to the medium and cooling. that for drug discovery, there is a 50 cost and time savings from target. Cultivation strategies and corresponding process costs covered a wide range of operational conditions, including different media, inducers, and temperatures. Data from batch bioreactor cultures were used as case study involving the production of pneumococcal surface protein A, a soluble recombinant protein, employing E. This paper presents a methodology to estimate and compare cost impacts related to utilities as well as medium composition, using simple design equations and accessible data. The impact of cultivation strategy on the cost of recombinant protein production is crucial for defining cost-effective bioreactor operation conditions.
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